Typical Application Cases and Troubleshooting Paths

Ahlstrom conjugate pad adoption

A colloidal gold project showed visible pad residue, weak T line and slightly red negative background. The first step was to keep NC membrane, absorbent pad, spraying amount and drying condition constant while comparing Ahlstrom 8964, 6613, 8951 and GL0194 candidate directions.

The goal was not simply fastest release, but a balance between controllable release, clean background and stable line shape. Treatment buffer, spraying amount, drying humidity and overlap should be recorded in one validation table.

High fluorescence background

In one fluorescence lateral flow direction, tube conjugates looked acceptable while negative-strip baseline increased after assembly. The troubleshooting route covered bead size, surface group, coupling ratio, blocking, conjugate pad release, low-background backing and reader parameters.

For FNBE fluorescent nanobead projects, useful records include particle size, surface group, coupling target, storage buffer, pad model, NC membrane, absorbent pad and reader settings.

Whole blood sample handling

Whole blood projects often face blood-cell interference, unstable flow, weak-positive suppression and background tailing. Filter material, sample pad treatment, sample volume, buffer system and absorbent driving force should be evaluated together.

GF2, GF4, whole blood filters, sample pads and absorbent pads need system matching. Before scale-up, weak-positive, negative, different hematocrit and lot-to-lot samples should be included.

Import substitution evaluation

When customers want shorter lead time or lower supply risk, substitution should not rely on model names alone. The current material, target material and candidate material should be compared under the same strip structure.

Evaluation should cover sensitivity, background, release, line shape, reading window, accelerated stability, lot consistency, packaging, documentation and long-term supply.

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